Human Primary Lung Endothelial Cells in Culture

Studies on the immunogenic properties of La Sota NDV propagated in embryo lung primary cells culture

Human endothelial cells in culture.

Daily changes of cultured endothelial cells were observed by phase contrast microscopy, time-lapse microcinematography and electron microscopy. By light microscopy, the cultured cells are polygonal in shape and form monolayered clusters. However, they become elongated like fibroblasts between 10 and 14 days. Proliferation of the cultured cells by mitotic division could not be recognized by cine...

Human Vascular Endothelial Cells in Culture

Human endothelial cells, obtained by collagenase treatment of term umbilical cord veins, were cultured using Medium 199 supplemented with 20% fetal calf serum. Small clusters of cells initially spread on plastic or glass, coalesced and grew to form confluent monolayers of polygonal cells by 7 days. Cells in primary and subcultures were identified as endothelium by the presence of Weibel-Palade ...

Endothelial Cells in Culture

The smooth muscle of the abdominal region of the chicken aorta derives from locally recruited mesenchyme (mesenchymal smooth muscle), whereas that of the thoracic region derives from the neural crest (ectomesenchymal smooth muscle). We hypothesized that this smooth muscle heterogeneity might affect important enzymatic functions of the vessel wall. Therefore, we measured angiotensin converting e...

Lung endothelial cells strengthen, but brain endothelial cells weaken barrier properties of a human alveolar epithelium cell culture model.

The blood-air barrier in the lung consists of the alveolar epithelium, the underlying capillary endothelium, their basement membranes and the interstitial space between the cell layers. Little is known about the interactions between the alveolar and the blood compartment. The aim of the present study was to gain first insights into the possible interplay between these two neighbored cell layers...